In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy.
Methods Mol Biol
; 1974: 21-30, 2019.
Article
em En
| MEDLINE
| ID: mdl-31098992
ABSTRACT
Discovery and development of gene targets for cancer therapeutics are lengthy and highly costly processes. Identification and evaluation of candidate gene targets are of fundamental importance. RNA interference allows candidate genes to be specifically and effectively knocked down in cancer cells. This tool can be easily incorporated into a loss-of-function approach in the initial evaluation of candidate gene targets for cancer treatment prior to moving on to animal studies and clinical trials. This chapter describes a relatively simple and straightforward protocol that makes use of small interfering RNA to achieve knockdown of the candidate gene target and to evaluate the resultant effects on four aspects of cancer cell behavior migration, invasion, proliferation, and adhesion.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
RNA Interferente Pequeno
/
Interferência de RNA
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Biologia Molecular
Tipo de estudo:
Guideline
Limite:
Female
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2019
Tipo de documento:
Article