Identifying the molecular and cellular signature of cardiac dilation following myocardial infarction.
Biochim Biophys Acta Mol Basis Dis
; 1865(7): 1845-1852, 2019 07 01.
Article
em En
| MEDLINE
| ID: mdl-31109452
ABSTRACT
Establishing molecular and cellular indicators that reflect the extent of dilation of the left ventricle (LV) after myocardial infarction (MI) may improve diagnostic and prognostic capabilities. We queried the Mouse Heart Attack Research Tool (mHART) 1.0 for day 7 post-MI mice (age 3-9â¯months, untreated males and females) with serial echocardiographic data at days 0, 1, and 7 (nâ¯=â¯51). Mice were classified into two subgroups determined by a median fold change of 1.6 in end-diastolic dimensions (EDD) normalized to pre-MI values; nâ¯=â¯26 fell below (moderate; mean of 1.42⯱â¯0.01) and nâ¯=â¯25 fell above this cut-off (extreme; mean of 1.79⯱â¯0.01; pâ¯<â¯0.001 vs. moderate). Plasma proteomic profiling of 34 analytes measured at day 7 post-MI from male mice (nâ¯=â¯12 moderate and 12 extreme) were evaluated as the test dataset, and receiver operating curve (ROC) analysis was used to assess strength of biomarkers. Females (nâ¯=â¯6 moderate and 9 extreme) were used as the validation dataset. Both by t-test and characteristic (ROC) curve analysis, lower macrophage inflammatory protein-1 gamma (MIP-1γ), lymphotactin, and granulocyte chemotactic protein-2 (GCP-2) were identified as plasma indicators for dilation status (pâ¯<â¯0.05 for all). Macrophage numbers were decreased and complement C5, laminin 1, and Ccr8 gene levels were significantly higher in the LV infarcts of the extreme dilation group (pâ¯<â¯0.05 for all). A composite panel including plasma MIP-1γ, lymphotactin, and GCP-2, and LV infarct Ccr8 and macrophage numbers strongly mirrored LV dilation status (AUCâ¯=â¯0.92; pâ¯<â¯0.0001). Using the mHART 1.0 database, we determined that a failure to mount sufficient macrophage-mediated inflammation was indicative of exacerbated LV dilation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cardiomiopatia Dilatada
/
Ventrículos do Coração
/
Infarto do Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta Mol Basis Dis
Ano de publicação:
2019
Tipo de documento:
Article