A general method to quantify ligand-driven oligomerization from fluorescence-based images.
Nat Methods
; 16(6): 493-496, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-31110281
ABSTRACT
Here, we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and the secretin receptor in the presence and absence of their agonist ligands. This method is fast and is scalable for high-throughput screening of drugs targeting protein-protein interactions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Receptores dos Hormônios Gastrointestinais
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Receptores Acoplados a Proteínas G
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Multimerização Proteica
/
Fluorescência
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Ano de publicação:
2019
Tipo de documento:
Article