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Preparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe.
Fouquet, Coralie; Trembleau, Alain.
Afiliação
  • Fouquet C; Sorbonne Université, CNRS, INSERM, Institut de Biologie Paris Seine, Laboratoire Neuroscience Paris Seine, F-75005 Paris, France.
  • Trembleau A; Sorbonne Université, CNRS, INSERM, Institut de Biologie Paris Seine, Laboratoire Neuroscience Paris Seine, F-75005 Paris, France.
Bio Protoc ; 9(8)2019 Apr 20.
Article em En | MEDLINE | ID: mdl-31131294
In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as the main steps of the procedure for axon tension measurement using the previously established Biomembrane Force Probe.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2019 Tipo de documento: Article