Measurement of factor VIII procoagulant antigen in normal subjects and in hemophilia A patients by an immunoradiometric assay and by an enzyme-linked immunosorbent assay.
Haemostasis
; 17(4): 173-81, 1987.
Article
em En
| MEDLINE
| ID: mdl-3114101
ABSTRACT
Factor VIII coagulant antigen (FVIIIAg) and FVIII coagulant activity (FVIIIC) were measured in 102 healthy individuals, in 5 hemophilia A carriers and in 21 hemophilia A patients before and after infusion of heat-treated high-purity FVIII concentrates. Factor VIIIAg was determined by a solid-phase micro enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies and by a conventional solid-phase immunoradiometric assay (IRMA). Factor VIIIC was assessed using a one-stage assay. The micro ELISA was decidedly more precise than the IRMA. There was a close correlation between the results obtained by the three assays in the plasma of healthy subjects and in hemophilia A carriers. After transfusion of FVIII concentrates to hemophilia A patients, the FVIIIAg recoveries were significantly lower than the FVIIIC recoveries and the biological half-life of FVIIIAg was significantly shorter than for FVIIIC. The calculated half-life of FVIIIC was longer than in any previous study.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator VIII
/
Hemofilia A
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Haemostasis
Ano de publicação:
1987
Tipo de documento:
Article