The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics.
Methods Mol Biol
; 1992: 201-214, 2019.
Article
em En
| MEDLINE
| ID: mdl-31148040
Fluorescence proteins changing spectral properties after exposure to light with a specific wavelength have recently become outstanding aids in the study of intracellular protein dynamics. Herein we show using Arabidopsis SYNAPTOTAGMIN 1 as a model protein that the Dendra2 green to red photoconvertible protein tag in combination with confocal scanning laser microscopy is a useful tool to study membrane protein intracellular dynamics.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Microscopia Confocal
/
Proteínas de Arabidopsis
/
Sinaptotagmina I
/
Corantes Fluorescentes
/
Membranas Intracelulares
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2019
Tipo de documento:
Article