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The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics.
Lesková, Alexandra; Kusá, Zuzana; Labajová, Mária; Krausko, Miroslav; Jásik, Ján.
Afiliação
  • Lesková A; Plant Science and Biodiversity Center, Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia.
  • Kusá Z; Plant Science and Biodiversity Center, Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia.
  • Labajová M; Plant Science and Biodiversity Center, Institute of Botany of the Czech Academy of Sciences, Slovak Academy of Sciences, Bratislava, Slovakia.
  • Krausko M; Plant Science and Biodiversity Center, Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia.
  • Jásik J; Plant Science and Biodiversity Center, Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia. jan.jasik@savba.sk.
Methods Mol Biol ; 1992: 201-214, 2019.
Article em En | MEDLINE | ID: mdl-31148040
Fluorescence proteins changing spectral properties after exposure to light with a specific wavelength have recently become outstanding aids in the study of intracellular protein dynamics. Herein we show using Arabidopsis SYNAPTOTAGMIN 1 as a model protein that the Dendra2 green to red photoconvertible protein tag in combination with confocal scanning laser microscopy is a useful tool to study membrane protein intracellular dynamics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Microscopia Confocal / Proteínas de Arabidopsis / Sinaptotagmina I / Corantes Fluorescentes / Membranas Intracelulares Idioma: En Revista: Methods Mol Biol Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Microscopia Confocal / Proteínas de Arabidopsis / Sinaptotagmina I / Corantes Fluorescentes / Membranas Intracelulares Idioma: En Revista: Methods Mol Biol Ano de publicação: 2019 Tipo de documento: Article