Allelic polymorphisms of dna repair genes and their influence on the formation of resistance to the development of bronchopulmonary pathology under the action of industrial aerosols.

ABSTRACT

OBJECTIVE: Introduction: The frequency of alleles and genotypes of DNA repair genes in people working due to the influence of industrial aerosols (miners and workers of asbestos-cement plants (n = 215)) was studied. The aim of the work was to identify allelic polymorphisms affecting the formation of resistance or leading to an increased risk of developing bronchopulmonary pathology. PATIENTS AND METHODS: Materials and methods: In 90 patients with bronchopulmonary pathology and 125 persons working under the same conditions but without respiratory system diseases, the polymerase chain reaction in real time was determined by the polymorphisms of DNA repair genes XPD (rs13181, rs799793), ERCC1 (rs11615), XRCC1 ( rs25487) and XRCC3 (rs861539), ATM (rs664677), XRCC7 (rs7003908) and MLH1 (rs1799977). RESULTS: Results: In the course of this study the alleles and genotypes contributing to resistance to the development of respiratory system pathologies were determined XRCC1•G/A (rs25487) (OR=0.57; 95% CI 0.32-1.02; P≤0.040; Χ²=4.14); MLH1•A (rs1799977) (OR=0.62; 95% CI 0.40-0.96; P≤0.020; Χ²=5.06); MLH1•A/A (rs1799977) (OR=0.43; 95% CI 0.24-0.79; P≤0.003; Χ²=8.73). Also, we established the alleles and genotypes associated with the risk of developing bronchopulmonary pathology XPD•C/C (rs13181) (OR=2.20, 95% CI 1.02-4.77; P≤0.020; Χ²=4.85); XRCC1•A/A (rs25487) (OR=3.37; 95 % CI 1.22-9.63; P≤0.008; Χ²=6.94); ATM•T/T (rs664677) (OR=2.48; 95% CI 1.16-5.31; Р≤0.010; Χ²=6.61); MLH1•G (rs1799977) (OR=1.61; 95% CI 1.04-2.49; P≤0.020; Χ²=5.06); MLH1•A/G (rs1799977) (OR=2.32; 95% CI 1.29-4.21; P≤0.002; Χ²=9.01). CONCLUSION: Conclusions: The results indicate the influence of allelic polymorphisms of DNA repair genes on the formation of resistance to the development of bronchopulmonary pathology under the action of industrial aerosols and open up prospects for the development of modern preventive measures.