Analysis of Post-Sample Collection EDTA Effects on Mean Platelet Volume Values in Relation to Overweight and Obese Patient Status.
Acta Haematol
; 142(3): 149-153, 2019.
Article
em En
| MEDLINE
| ID: mdl-31189163
ABSTRACT
BACKGROUND:
Mean platelet volume (MPV) may be a useful biomarker for platelet activation in obese patients. However, storage duration and use of anticoagulant K3-ethylenediaminetetraacetic acid (EDTA) may interfere with these measurements.OBJECTIVES:
The aim of this study was to measure the variability of MPV in obese patients following exposure to EDTA.METHOD:
A total of 160 patients were divided into 3 groups according to body mass index (BMI; normal <25 kg/m2; overweight 25-30 kg/m2; obese >30 kg/m2). Blood was collected in sterile tubes containing K3-EDTA. Blood cell counts were obtained using the CELL-DYN Ruby system immediately and 1, 2, and 3 h after collection.RESULTS:
MPV was found to be directly proportional to BMI. With the addition of EDTA, MPV was increased in the first hour after collection; MPV then decreased to levels that were lower than initial baseline measurements.CONCLUSIONS:
K3-EDTA use alters platelet volume. The time from collection to measurement should be standardized to reduce MPV value variance. MPV should be determined within 1 h of collection to avoid anticoagulant use-related interference.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Plaquetas
/
Ácido Edético
/
Volume Plaquetário Médio
/
Obesidade
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Acta Haematol
Ano de publicação:
2019
Tipo de documento:
Article