Xenobiotic Response Elements (XREs) from Human CYP1A1 Gene Enhance the hTERT Promoter Activity.
Dokl Biochem Biophys
; 485(1): 150-152, 2019 Mar.
Article
em En
| MEDLINE
| ID: mdl-31201638
ABSTRACT
A hybrid 6XRE-hTERT promoter consisting of the hTERT tumor-specific promoter and six copies of the XRE element from the CYP1A1 human gene promoter was created. Using a human lung cancer cells as a model, we showed that XRE elements in the hybrid promoter greatly increase the activity of the hTERT promoter and ensure the reporter gene transcriptional activation in response to the treatment of the cells with the AhR ligand benzo(a)pyrene. However, similar effects were also observed in normal human bronchial epithelial cells HBEpC, which indicates the loss of the tumor-specific activity by the 6XRE-hTERT hybrid promoter. XRE elements can be used for nonspecific transcription enhancement but are unsuitable for the creation of tumor-specific promoters with enhanced activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Transcricional
/
Telomerase
/
Citocromo P-450 CYP1A1
/
Elementos de Resposta
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Dokl Biochem Biophys
Ano de publicação:
2019
Tipo de documento:
Article