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Evidence of nitrite acting as a stable and robust inducer of non-cultivability in Mycobacterium tuberculosis with physiological relevance.
Gample, Suwarna P; Agrawal, Sonia; Sarkar, Dhiman.
Afiliação
  • Gample SP; CSIR-National Chemical Laboratory, Organic Chemistry Division, Pune, 411008, Maharashtra, India.
  • Agrawal S; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
  • Sarkar D; CSIR-National Chemical Laboratory, Organic Chemistry Division, Pune, 411008, Maharashtra, India.
Sci Rep ; 9(1): 9261, 2019 06 25.
Article em En | MEDLINE | ID: mdl-31239517
Tuberculosis (TB) is the ninth leading cause of death worldwide, ranking above human immunodeficiency virus. Latency is the major obstacle in the eradication of this disease. How the physiology of the pathogen changes in transition to the latent stage needs to be understood. The latent bacteria extracted from animal hosts exist in a nonculturable (NC) phase, whereas bacteria extracted from most in vitro models are culture-positive. In the present study, we observed that nitrite, up to a concentration of 5 mM, shows the growth of Mycobacterium tuberculosis (MTB) in liquid media, but this effect starts reversing at higher concentrations. At a concentration of 10 mM, nitrite induces rapid nonculturability of MTB at the aerobic stage. This noncultivable dormancy was confirmed by analyzing the characteristics of NC bacteria. Further differential gene expression analyses clearly supported the formation of a dormancy phenotype. This study will be helpful for the use of this bacillus as a dormancy model in future studies on TB latency.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 / 3_ND / 4_TD Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Tuberculose / Regulação Bacteriana da Expressão Gênica / Tuberculose Latente / Mycobacterium tuberculosis / Nitritos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 / 3_ND / 4_TD Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Tuberculose / Regulação Bacteriana da Expressão Gênica / Tuberculose Latente / Mycobacterium tuberculosis / Nitritos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article