Underestimated risk from ozonation of wastewater containing bromide: Both organic byproducts and bromate contributed to the toxicity increase.
Water Res
; 162: 43-52, 2019 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-31254885
ABSTRACT
Ozonation is widely used in wastewater treatment but the associated byproduct formation is a concern. When ozonation is used in the presence of bromide, bromate is generally considered as a major byproduct, and few studies have examined the toxicity of organic byproducts. This study was designed to investigate the cytotoxicity, genotoxicity and DNA/RNA oxidative damage to Chinese hamster ovary (CHO) cells of organic extracts from ozonated wastewater in the absence or presence of bromide. Ozonation effectively detoxified secondary effluents containing no bromide. However, ozonation significantly increased the cytotoxicity and genotoxicity of the effluents spiked with a bromide concentration as low as 100⯵g/L, compared with the bromide-free effluent. When the bromide concentration in the effluent was increased to 2000⯵g/L, ozonation resulted in 1.4-1.5 times the cytotoxicity and 1.5-5.0 times the genotoxicity of the non-ozonated secondary effluent. Besides, the oxidative stress (including reactive oxygen species and reactive nitrogen species) and DNA/RNA oxidative damage also became more severe and a high level of 8-hydroxy-(deoxy)guanosine was detected in the CHO cell nucleus in the presence of bromide. Cytotoxicity and genotoxicity were found to increase with the formation of total organic bromine (TOBr). When the CHO cells were exposed to both the organic byproducts and bromate formed from wastewater containing 500 and 2000⯵g/L bromide, bromate significantly increased oxidative stress and DNA/RNA oxidative damage at relatively high concentration factors, suggesting both organic byproduct and bromate can contribute to toxicity increase. During ozonation of the effluent containing bromide, particular attention should be paid to the organic byproducts such as TOBr.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ozônio
/
Poluentes Químicos da Água
/
Purificação da Água
Tipo de estudo:
Etiology_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Water Res
Ano de publicação:
2019
Tipo de documento:
Article