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[Effects of MD2 gene silencing on high glucose-induced proliferation inhibition, apoptosis and inflammation in rat cardiomyocytes].
Lin, Zhong-Min; Chen, Guo-Rong; Zhang, Quan-Bo; Wang, Fang; Xiang, Lan-Ting; Cao, Qiong-Jie.
Afiliação
  • Lin ZM; Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Chen GR; Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Zhang QB; Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Wang F; Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Xiang LT; Department of Pathology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Cao QJ; State Key Laboratory of Ophthalmology, Optometry and Vision Science, Eye Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(3): 273-278, 2019 May 28.
Article em Zh | MEDLINE | ID: mdl-31257812
ABSTRACT

OBJECTIVE:

To investigate the effects of myeloid differentiation-2 (MD2) gene silencing on high glucose-induced proliferation inhibition, apoptosis and inflammation in rat cardiomyocytes.

METHODS:

The immortalized rat cardiomyocyte cell line H9C2 were transfected with MD2 small interfering RNA (si-MD2) and negative control for 24 h, then stimulated with high glucose (HG) for 48 h. RT-qPCR was performed to detect the mRNA levels of MD2 and inflammatory factors TNF-α, IL-1ß and IL-6. MTS and flow cytometry were used to evaluate cell proliferation, cell cycle and apoptosis rate. Western blot was used to detect protein expression levels and phosphorylation levels.

RESULTS:

The mRNA and protein levels of MD2 in H9C2 cells were dramatically decreased after transfected with si-MD2 (P<0.01). After stimulation of high glucose, the mRNA levels of inflammatory factors, the cells in G0/G1 phase , the cell apoptosis rate and the protein level of cleaved Caspase-3 were significantly increased, while the cell proliferation ability was decreased (P<0.01). MD2 gene silencing antagonized the effects of high glucose on cell proliferation, cell cycle, cell apoptosis and the mRNA levels of TNF-α, IL-1ß , IL-6(P<0.05). Western blot analysis showed that the phosphorylation levels of extracellular signal-regulated kinase(ERK1/2), P38 mitogen-activated protein kinase(P38 MAPK) and C-Jun N-terminal kinase(JNK) protein were increased significantly in H9C2 cells treated with high glucose, which could be reversed by silencing of MD2 (P<0.01).

CONCLUSION:

This study demonstrates that MD2 gene silencing reverses high glucose-induced myocardial inflammation, apoptosis and proliferation inhibition via the mechanisms involving suppression of ERK, P38 MAPK, JNK signaling pathway.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Inativação Gênica / Miócitos Cardíacos / Proliferação de Células / Antígeno 96 de Linfócito Limite: Animals Idioma: Zh Revista: Zhongguo Ying Yong Sheng Li Xue Za Zhi Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Inativação Gênica / Miócitos Cardíacos / Proliferação de Células / Antígeno 96 de Linfócito Limite: Animals Idioma: Zh Revista: Zhongguo Ying Yong Sheng Li Xue Za Zhi Ano de publicação: 2019 Tipo de documento: Article