Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway.
Mar Drugs
; 17(7)2019 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-31277207
ABSTRACT
The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Transdução de Sinais
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Glucana 1,4-alfa-Glucosidase
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Proteínas Proto-Oncogênicas c-akt
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Células Progenitoras Endoteliais
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Mar Drugs
Ano de publicação:
2019
Tipo de documento:
Article