A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics.
J Microbiol Methods
; 164: 105660, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31301322
ABSTRACT
An inexpensive method for determining minimum inhibitory concentrations (MIC) using ink-jet printing to deposit drug solutions and bacterial suspensions onto agar was developed. Substrate concentrations were varied using a "Y-value", whereby a series of rectangles with the same width and colour but different heights were printed within a fixed unit area. Prior to MIC determination, the printer cartridges used were calibrated using Fast Green dye. The impact of thermal ink-jet printing on bacterial viability was assessed by colony counting and found not to be deleterious. MIC determinations were conducted by printing varying concentrations of the antibiotics onto agar-coated glass slides then printing a thin even film of a known bacterial density of Lactobacillus acidophilus. Broth microdilution was performed simultaneously to validate the results. Slides and well plates were then incubated anaerobically for 48â¯h. The MIC values obtained for the antibiotics used were within a permissible range for comparison.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Testes de Sensibilidade Microbiana
/
Impressão Tridimensional
/
Antibacterianos
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2019
Tipo de documento:
Article