Self-Assembly and Biogenesis of the Cellular Membrane are Dictated by Membrane Stretch and Composition.
J Phys Chem B
; 123(32): 6997-7005, 2019 08 15.
Article
em En
| MEDLINE
| ID: mdl-31322890
ABSTRACT
The cell plasma membrane is a highly dynamic organelle governing a wide range of cellular activities including ion transport, secretion, cell division, growth, and development. The fundamental process involved in the addition of new membranes to pre-existing plasma membranes, however, is unclear. Here, we report, using biophysical, morphological, biochemical, and molecular dynamic simulations, the selective incorporation of proteins and lipids from the cytosol into the cell plasma membrane dictated by membrane stretch and composition. Stretching of the cell membrane as a consequence of volume increase following incubation in a hypotonic solution and results in the incorporation of cytosolic proteins and lipids into the existing plasma membrane. Molecular dynamic simulations further confirm that increased membrane stretch results in the rapid insertion of lipids into the existing plasma membrane. Similarly, depletion of cholesterol from the cell plasma membrane selectively alters the incorporation of lipids into the membrane.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Membrana Celular
/
Citosol
/
Eritrócitos
/
Insulinoma
/
Lipídeos de Membrana
Limite:
Animals
Idioma:
En
Revista:
J Phys Chem B
Ano de publicação:
2019
Tipo de documento:
Article