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Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.
Boassa, Daniela; Lemieux, Sakina P; Lev-Ram, Varda; Hu, Junru; Xiong, Qing; Phan, Sebastien; Mackey, Mason; Ramachandra, Ranjan; Peace, Ryan Emily; Adams, Stephen R; Ellisman, Mark H; Ngo, John T.
Afiliação
  • Boassa D; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: dboassa@ucsd.edu.
  • Lemieux SP; Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
  • Lev-Ram V; Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
  • Hu J; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA.
  • Xiong Q; Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
  • Phan S; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA.
  • Mackey M; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA.
  • Ramachandra R; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA.
  • Peace RE; Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA 02215, USA.
  • Adams SR; Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
  • Ellisman MH; Department of Neurosciences, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
  • Ngo JT; Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA 02215, USA. Electronic address: jtngo@bu.edu.
Cell Chem Biol ; 26(10): 1407-1416.e5, 2019 10 17.
Article em En | MEDLINE | ID: mdl-31378710
ABSTRACT
A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as "multicolor" electron microscopy (EM) via the photooxidation of 3-3'-diaminobenzidine and its derivatives.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Arabidopsis / Flavoproteínas / Proteínas Luminescentes Limite: Humans Idioma: En Revista: Cell Chem Biol Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Arabidopsis / Flavoproteínas / Proteínas Luminescentes Limite: Humans Idioma: En Revista: Cell Chem Biol Ano de publicação: 2019 Tipo de documento: Article