Characterizing differences in the collagen fiber organization of skin wounds using quantitative polarized light imaging.
Wound Repair Regen
; 27(6): 711-714, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31418977
ABSTRACT
Collagen fiber organization requires characterization in many biomedical applications, but it is difficult to objectively quantify in standard histology tissue sections. Quantitative polarized light imaging is a low-cost technique that allows for rapid measurement of collagen fiber orientation and thickness. In this study, we utilize a quantitative polarized light imaging system to characterize fiber orientation and thickness from wound sections. Full thickness skin wound sections that were previously stained with hematoxylin and eosin were used to assess collagen fiber content and organization at different points during the wound healing process. Overall, wounds exhibited a measurable increase in collagen fiber thickness and a nonlinear change in fiber reorganization within the wound. Our study demonstrates that quantitative polarized light imaging is an inexpensive alternative or supplement to standard histology protocols, requiring no additional stains or dyes, and yields repeatable quantitative assessments of collagen organization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Ferimentos e Lesões
/
Processamento de Imagem Assistida por Computador
/
Colágeno
/
Microscopia de Polarização
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Wound Repair Regen
Ano de publicação:
2019
Tipo de documento:
Article