Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins.

Kohno, Takao; Ogino, Himari; Yamakage, Yuko; Hattori, Mitsuharu

Kohno, Takao; Ogino, Himari; Yamakage, Yuko; Hattori, Mitsuharu.

Afiliação

Kohno T; Department of Biomedical Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan.
Ogino H; Department of Biomedical Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan.
Yamakage Y; Department of Biomedical Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan.
Hattori M; Department of Biomedical Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi, Japan. mhattori@phar.nagoya-cu.ac.jp.

Methods Mol Biol ; 2043: 93-104, 2020.

Article em En | MEDLINE | ID: mdl-31463905

ABSTRACT

ABSTRACT

Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins.

Assuntos

Proteínas ADAMTS/genética; Moléculas de Adesão Celular Neuronais/genética; Proteínas da Matriz Extracelular/genética; Expressão Gênica; Proteínas do Tecido Nervoso/genética; Pró-Colágeno N-Endopeptidase/genética; Serina Endopeptidases/genética; Proteínas ADAMTS/metabolismo; Moléculas de Adesão Celular Neuronais/metabolismo; Proteínas da Matriz Extracelular/metabolismo; Células HEK293; Humanos; Proteínas do Tecido Nervoso/metabolismo; Pró-Colágeno N-Endopeptidase/metabolismo; Engenharia de Proteínas; Proteínas Recombinantes/metabolismo; Proteína Reelina; Serina Endopeptidases/metabolismo

Palavras-chave

Expi293; HEK293T; Lipofection; SDS-PAGE; Western blotting

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pró-Colágeno N-Endopeptidase / Serina Endopeptidases / Moléculas de Adesão Celular Neuronais / Expressão Gênica / Proteínas da Matriz Extracelular / Proteínas ADAMTS / Proteínas do Tecido Nervoso Limite: Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2020 Tipo de documento: Article

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