Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins.
Methods Mol Biol
; 2043: 93-104, 2020.
Article
em En
| MEDLINE
| ID: mdl-31463905
ABSTRACT
Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pró-Colágeno N-Endopeptidase
/
Serina Endopeptidases
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Moléculas de Adesão Celular Neuronais
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Expressão Gênica
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Proteínas da Matriz Extracelular
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Proteínas ADAMTS
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Proteínas do Tecido Nervoso
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2020
Tipo de documento:
Article