Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell-Mediated Mechanism.
JACC Basic Transl Sci
; 4(4): 480-494, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31468003
ABSTRACT
Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA.
ATP, adenosine triphosphate; CVD, cardiovascular disease; DC, dendritic cell; GM-CSF, granulocyte-macrophage colony-stimulating factor; HLA, human leukocyte antigen; HSA, human serum albumin; HSP, heat shock protein; IFN, interferon; IL, interleukin; IgM, immunoglobulin M; LDL, low-density lipoprotein; MDA, malondialdehyde; MS, mass spectrometry; OxLDL, oxidized low-density lipoprotein; PCR, polymerase chain reaction; T cells; TCR, T-cell receptor; TGF, transforming growth factor; TLR, Toll-like receptor; TNF, tumor necrosis factor; atherosclerosis; dendritic cells; malondialdehyde; oxidized low-density lipoprotein
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
JACC Basic Transl Sci
Ano de publicação:
2019
Tipo de documento:
Article