Identification and Characterization of a Metalloprotein Involved in Gallium Internalization in Pseudomonas aeruginosa.
ACS Infect Dis
; 5(10): 1693-1697, 2019 10 11.
Article
em En
| MEDLINE
| ID: mdl-31475514
ABSTRACT
Gallium nitrate (Ganite) is a potential drug for the treatment of Pseudomonas aeruginosa infection. CRISPR/Cas9-based gene mutagenesis studies reveal that siderophore pyochelin-facilitated uptake and an ABC transporter are two major Ga3+ internalization pathways in Pseudomonas aeruginosa (P. aeruginosa). Crystal structures reveal that Ga3+ and Fe3+ occupy exactly the same metal site of HitA, a periplasmic iron-binding protein of the ABC transporter system. The study provides a molecular basis for Ga3+ internalization by P. aeruginosa and facilitates gallium-based antimicrobial drug development.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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Gálio
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Metaloproteínas
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Antibacterianos
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Revista:
ACS Infect Dis
Ano de publicação:
2019
Tipo de documento:
Article