Development and validation of LC-MS/MS method for determination of DNDI-VL-2098 in mouse, rat, dog and hamster blood.
Bioanalysis
; 11(15): 1419-1435, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31490107
ABSTRACT
Aim:
To develop a bioanalytical method to support pharmacokinetic evaluation of DNDI-VL-2098 in mouse, rat, dog and hamster following oral administration. Results &methodology:
A robust LC-MS/MS bioanalytical method was developed to quantify DNDI-VL-2098. DNDI-VL-2098 showed time-dependent recovery loss in acetonitrile precipitated plasma in all species. Acid-lysed whole blood was identified as a matrix in which recovery was stable over time. A two-step extraction procedure was used, with protein precipitation followed by liquid-liquid extraction with methyl tert-butyl ether. The assay was validated in the dynamic range of 5-5000 ng/ml for mouse, rat and dog blood, and a fit-for-purpose method was developed for hamster.Conclusion:
A specific LC-MS/MS assay for DNDI-VL-2098 was developed and validated in hemolyzed blood.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxazóis
/
Análise Química do Sangue
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Nitroimidazóis
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2019
Tipo de documento:
Article