In- vitro evaluation of the bioactivity and the biocompatibility of a novel coated UHMWPE biomaterial for biomedical applications.

ABSTRACT

Ultra-High Molecular Weight Polyethylene (UHMWPE) is the gold standard biomaterial used as a bearing surface in total joint replacement surgeries. However, osteolysis and subsequent implant failure as a result of the production of wear debris may occur at the contacting surfaces. One potential solution to overcome this problem is to strengthen the surface of UHMWPE which can be achieved by adding a thin coating layer made of Polyamide. In this article, a combination of biological and biochemical tests, including cell viability, antibacterial activity (using Escherichia coli and Staphylococcus aureus), and wound healing assays were performed to assess the bioactivity and the biocompatibility of the coated specimens. Additional tests, such as simulated body fluid absorption, Alizarin Red Staining, Scanning Electron Microscopy, Energy-dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy techniques were conducted to evaluate the moisture uptake, osteogenic activity, and the morphology of the coated samples. The antibacterial activity test results after 24 h incubation showed that the nylon-coated UHMWPE has significantly higher antibacterial activity compared to the uncoated UHMWPE. The results of wound closure showed that nylon-coated UHMWPE promotes more wound healing compared to the uncoated material that exhibits a similar percentage of wound closure as the control. This is the first study to demonstrate the superiority of the proposed coated biomaterial for wound healing applications with improved antibacterial capabilities.