Polymicrobial oral biofilm models: simplifying the complex.
J Med Microbiol
; 68(11): 1573-1584, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31524581
ABSTRACT
Over the past century, numerous studies have used oral biofilm models to investigate growth kinetics, biofilm formation, structure and composition, antimicrobial susceptibility and host-pathogen interactions. In vivo animal models provide useful models of some oral diseases; however, these are expensive and carry vast ethical implications. Oral biofilms grown or maintained in vitro offer a useful platform for certain studies and have the advantages of being inexpensive to establish and easy to reproduce and manipulate. In addition, a wide range of variables can be monitored and adjusted to mimic the dynamic environmental changes at different sites in the oral cavity, such as pH, temperature, salivary and gingival crevicular fluid flow rates, or microbial composition. This review provides a detailed insight for early-career oral science researchers into how the biofilm models used in oral research have progressed and improved over the years, their advantages and disadvantages, and how such systems have contributed to our current understanding of oral disease pathogenesis and aetiology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Biofilmes
/
Boca
Tipo de estudo:
Prognostic_studies
Aspecto:
Ethics
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2019
Tipo de documento:
Article