Structural and functional aspects of mannuronic acid-specific PL6 alginate lyase from the human gut microbe <i>Bacteroides cellulosilyticus</i>.

Stender, Emil G P; Dybdahl Andersen, Christian; Fredslund, Folmer; Holck, Jesper; Solberg, Amalie; Teze, David; Peters, Günther H J; Christensen, Bjørn E; Aachmann, Finn L; Welner, Ditte H; Svensson, Birte

Structural and functional aspects of mannuronic acid-specific PL6 alginate lyase from the human gut microbe Bacteroides cellulosilyticus.

Stender, Emil G P; Dybdahl Andersen, Christian; Fredslund, Folmer; Holck, Jesper; Solberg, Amalie; Teze, David; Peters, Günther H J; Christensen, Bjørn E; Aachmann, Finn L; Welner, Ditte H; Svensson, Birte.

Afiliação

Stender EGP; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Dybdahl Andersen C; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Fredslund F; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Holck J; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Solberg A; Department of Biotechnology and Food Science, NTNU, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Teze D; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Peters GHJ; Department of Chemistry, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Christensen BE; Department of Biotechnology and Food Science, NTNU, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Aachmann FL; Department of Biotechnology and Food Science, NTNU, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Welner DH; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Svensson B; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark bis@bio.dtu.dk.

J Biol Chem ; 294(47): 17915-17930, 2019 11 22.

Article em En | MEDLINE | ID: mdl-31530640

Assuntos

Bacteroides/enzimologia; Microbioma Gastrointestinal; Ácidos Hexurônicos/metabolismo; Polissacarídeo-Liases/química; Polissacarídeo-Liases/metabolismo; Alginatos/química; Alginatos/metabolismo; Bacteroides/genética; Genoma Bacteriano; Humanos; Cinética; Simulação de Acoplamento Molecular; Proteínas Mutantes/química; Proteínas Mutantes/metabolismo; Mutação/genética; Estrutura Secundária de Proteína; Eletricidade Estática; Homologia Estrutural de Proteína; Relação Estrutura-Atividade; Especificidade por Substrato

Palavras-chave

Bacteroides cellulolyticus; alginate lyase; asparagine ladder; crystal structure; enzyme kinetics; enzyme mechanism; enzyme mutation; imidazole rescue; molecular docking; parallel ß-helix

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Polissacarídeo-Liases / Bacteroides / Microbioma Gastrointestinal / Ácidos Hexurônicos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2019 Tipo de documento: Article

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