Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization.
Nat Commun
; 10(1): 4456, 2019 10 01.
Article
em En
| MEDLINE
| ID: mdl-31575859
ABSTRACT
Fertilization is essential for species survival. Although Izumo1 and Juno are critical for initial interaction between gametes, additional molecules necessary for spermegg fusion on both the sperm and the oocyte remain to be defined. Here, we show that phosphatidylserine (PtdSer) is exposed on the head region of viable and motile sperm, with PtdSer exposure progressively increasing during sperm transit through the epididymis. Functionally, masking phosphatidylserine on sperm via three different approaches inhibits fertilization. On the oocyte, phosphatidylserine recognition receptors BAI1, CD36, Tim-4, and Mer-TK contribute to fertilization. Further, oocytes lacking the cytoplasmic ELMO1, or functional disruption of RAC1 (both of which signal downstream of BAI1/BAI3), also affect sperm entry into oocytes. Intriguingly, mammalian sperm could fuse with skeletal myoblasts, requiring PtdSer on sperm and BAI1/3, ELMO2, RAC1 in myoblasts. Collectively, these data identify phosphatidylserine on viable sperm and PtdSer recognition receptors on oocytes as key players in spermegg fusion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Fagócitos
/
Fosfatidilserinas
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Interações Espermatozoide-Óvulo
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Espermatozoides
Limite:
Animals
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Female
/
Humans
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Male
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2019
Tipo de documento:
Article