A label-free fluorescent enhancement nanosensor for ultrasensitive and highly selective detection of miRNA-378 through signal synergy amplification.
Anal Chim Acta
; 1087: 86-92, 2019 Dec 09.
Article
em En
| MEDLINE
| ID: mdl-31585570
ABSTRACT
For early detection and diagnosis of gastric cancer, a novel, highly sensitive detection of microRNA-378 was developed through rolling circle amplification and DNA-templated silver nanoclusters as a lable-free fluorescent probe. DNA-templated fluorescent silver nanoclusters (DNA/AgNCs) to make target signal cascade amplification were prepared and identified through their fluorescent spectrum. MiRNA-378 trigged rolling circle amplification to obtain the complementary sequence (cDNA) to combine with two DNA/AgNCs in the middle of a sandwich structure to induce the new fluorescent signal at a new wavelength. In the presence of microRNA-378, a large amount of RCA product cDNAs were hybridized with DNA/AgNCs as the fluorescence nanocluster beacon, resulting in fluorescence enhanced "turn-on" phenomenon. This study indicated that amplified fluorescence detection of microRNA-378 is a stable, low-cost, highly specific, and ultra-low as 1.07â¯fM detectability. The proposed approach of signal synergetic amplification facilitating the fluorescence detection microRNA shows great potential for potentially clinically diagnosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Biomarcadores Tumorais
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MicroRNAs
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Nanopartículas Metálicas
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Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2019
Tipo de documento:
Article