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Molecular and computational analysis of Ghrelin, growth hormone Secretagogues receptor and mRNA expression of Growth-related genes after exogenous administered ghrelin peptide in Labeo rohita.
Dar, Showkat Ahmad; Srivastava, Prem Prakash; Rather, Mohd Ashraf; Varghese, Tincy; Rasool, Sheikh Irfan; Gupta, Subodh.
Afiliação
  • Dar SA; Division of Fish Nutrition, Physiology, and Biochemistry, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
  • Srivastava PP; Division of Fish Nutrition, Physiology, and Biochemistry, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India. Electronic address: ppsrivastava@cife.edu.in.
  • Rather MA; Fish Biotechnology Laboratory, Department of Fisheries Biology, College of Fisheries, Ratnagiri, Maharashtra 415629, India.
  • Varghese T; Division of Fish Nutrition, Physiology, and Biochemistry, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
  • Rasool SI; Division of Fish Nutrition, Physiology, and Biochemistry, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
  • Gupta S; Division of Fish Nutrition, Physiology, and Biochemistry, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Int J Biol Macromol ; 142: 756-768, 2020 Jan 01.
Article em En | MEDLINE | ID: mdl-31622719
Ghrelin is a peptide hormone secreted primarily by the stomach and is involved in controlling growth by governing different functions in vertebrates including feed intake and metabolism in vertebrates. This work was aimed to identify sequences of ghrelin gene and growth hormone secretagogue receptor (GHSR) in Labeo rohita. The full-length cDNA sequence of ghrelin is 453 bp including 5'-untranslated region (UTR) of 65 bp, 3'-UTR of 76 bp with a poly-A frame. An open reading frame (ORF) is 312 bp, which encodes a peptide of 103 amino acid residues. A secondary structure of GHSR protein consists of alpha helix 66.0%, 16% disordered and 43% transmembrane helix. Molecular docking and interaction between synthetic ghrelin peptides and GHSR in the contact map revealed 19 amino acid residues closer than 4.5 Šdistance. The mRNA expression level of ghrelin, leptin, GHSR, growth hormone releasing hormone (GHRH) and insulin growth factor-I (IGF-1) revealed significant changes (p < 0.05), in the different treatments. The outcome of the present work contributes to understanding the role of ghrelin and its mechanism of action in regulating the expression of growth-related genes and feed intake in fish.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Grelina / Receptores de Grelina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Grelina / Receptores de Grelina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2020 Tipo de documento: Article