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Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein.
Choi, Go-Woon; Oh, June-Pyo; Cho, In-Sook; Ju, Hye-Kyoung; Hu, Wen-Xing; Kim, Boram; Seo, Eun-Young; Park, Jong-Seok; Domier, Leslie L; Hammond, John; Song, Kihak; Lim, Hyoun-Sub.
Afiliação
  • Choi GW; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Oh JP; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Cho IS; Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea.
  • Ju HK; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Hu WX; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Kim B; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Seo EY; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Park JS; Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
  • Domier LL; Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Hammond J; United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA.
  • Song K; Department of Urology, Chungnam National University School of Medicine, Daejeon 34134, Korea.
  • Lim HS; Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
Plant Pathol J ; 35(5): 538-542, 2019 Oct.
Article em En | MEDLINE | ID: mdl-31632228
In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Plant Pathol J Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Plant Pathol J Ano de publicação: 2019 Tipo de documento: Article