Investigation of the pharmacodynamic substances in dahuang zhechong pill that inhibit energy metabolism.
J Ethnopharmacol
; 251: 112332, 2020 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-31669443
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Dahuang Zhechong pill (DHZCP) is a commonly used traditional Chinese medicine for the treatment of hepatocarcinoma. AIM OF THE STUDY Previous studies have found that DHZCP can exert anti-hepatocarcinoma effects and reverse drug resistance by inhibiting energy metabolism. The goal of this study was to further explore the pharmacodynamic substances that inhibit energy metabolism. METHODS:
The components of DHZCP absorbed into plasma were identified by UHPLC-Q-TOF-MS/MS. The Swiss and STITCH databases were used for target collection. The DAVID database was used for pathway enrichment analysis. Cytoscape software was used for network construction. The CCK-8 method detected cell viability. Chemiluminescence was used to detect ATP levels.RESULTS:
A total of 89 components absorbed into plasma were identified by UHPLC-Q-TOF-MS/MS. Based on this, 24 potential pharmacodynamic substances were selected by network pharmacology. Among them, 11 components such as rhein can significantly inhibit ATP levels.CONCLUSIONS:
Rhein, emodin, chrysophanol, hypoxanthine, baicalein, baicalin, wogonoside, acteoside, formononetin, isoliquiritigenin, and glycyrrhizic acid were the pharmacodynamic substances responsible for inhibition of energy metabolism of DHZCP.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Metabolismo Energético
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Compostos Fitoquímicos
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2020
Tipo de documento:
Article