Your browser doesn't support javascript.
loading
EBV-miR-BHRF1-1 Targets p53 Gene: Potential Role in Epstein-Barr Virus Associated Chronic Lymphocytic Leukemia.
Xu, Dan-Min; Kong, Yi-Lin; Wang, Li; Zhu, Hua-Yuan; Wu, Jia-Zhu; Xia, Yi; Li, Yue; Qin, Shu-Chao; Fan, Lei; Li, Jian-Yong; Liang, Jin-Hua; Xu, Wei.
Afiliação
  • Xu DM; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
  • Kong YL; Key Laboratory of Hematology of Nanjing Medical University, Nanjing, China.
  • Wang L; Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, China.
  • Zhu HY; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
  • Wu JZ; Key Laboratory of Hematology of Nanjing Medical University, Nanjing, China.
  • Xia Y; Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, China.
  • Li Y; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
  • Qin SC; Key Laboratory of Hematology of Nanjing Medical University, Nanjing, China.
  • Fan L; Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, China.
  • Li JY; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
  • Liang JH; Key Laboratory of Hematology of Nanjing Medical University, Nanjing, China.
  • Xu W; Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, China.
Cancer Res Treat ; 52(2): 492-504, 2020 Apr.
Article em En | MEDLINE | ID: mdl-31671936
PURPOSE: The purpose of this study was to investigate the prognostic impact of Epstein-Barr virus (EBV)-microRNA (miRNA, miR)-BHRF1-1 with chronic lymphocytic leukemia (CLL) as well as role of EBV-miR-BHRF1-1 in p53 gene. MATERIALS AND METHODS: Quantitative reverse transcription-polymerase chain reaction and western blotting were used to quantify EBV-miR-BHRF1-1 and p53 expression in cultured CLL. RESULTS: p53 aberration was associated with the higher expression level of EBV-miR-BHRF1-1 (p < 0.001) which was also an independent prognostic marker for overall survival (p=0.028; hazard ratio, 5.335; 95% confidence interval, 1.193 to 23.846) in 97 newly-diagnosed CLL patients after adjusted with International Prognostic Index for patients with CLL. We identified EBV-miR-BHRF1-1 as a viral miRNA regulator of p53. EBV-miR-BHRF1-1 repressed luciferase reporter activity by specific interaction with the seed region within the p53 3'- untranslated region. Discordance of p53 messenger RNA and protein expression was associated with high EBV-miR-BHRF1-1 levels in CLL patients and cell lines. EBV-miR-BHRF1- 1 inhibition upregulated p53 protein expression, induced cell cycle arrest and apoptosis and decreased cell proliferation in cell lines. EBV-miR-BHRF1-1 mimics downregulated p53 protein expression, decreased cell cycle arrest and apoptosis, and induced cell proliferation in cell lines. CONCLUSION: This study supported the role of EBV-miR-BHRF1-1 in p53 regulation in vitro. Our results support the potential of EBV-miR-BHRF1-1 as a therapeutic target in EBV-associated CLL with p53 gene aberration.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Leucemia Linfocítica Crônica de Células B / Genes p53 / Infecções por Vírus Epstein-Barr Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Res Treat Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Leucemia Linfocítica Crônica de Células B / Genes p53 / Infecções por Vírus Epstein-Barr Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Res Treat Ano de publicação: 2020 Tipo de documento: Article