Tetrabromoethylcyclohexane (TBECH) exhibits immunotoxicity in murine macrophages.
Environ Toxicol
; 35(2): 159-166, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-31696622
ABSTRACT
Tetrabromoethylcyclohexane (TBECH) has been linked to endocrine disruption, hepatotoxicity, and reproductive toxicity. However, its immunotoxicity remains largely unknown. In the present study, RAW 264.7 cells, mouse macrophage cell line, were exposed to TBECH. MTT assays showed that TBECH significantly enhanced lactate dehydrogenase (LDH) release in RAW 264.7 cells. The mRNA expression of some proapoptotic genes was upregulated by TBECH. Accordingly, TBECH elevated caspase-3 activity. In addition, TBECH upregualted the mRNA levels of some pro-inflammatory cytokines, whereas it downregulated LPS-stimulated mRNA expression of these cytokines. Moreover, TBECH downregulated the mRNA expression of selected antigen presenting-related genes. Furthermore, TBECH increased reactive oxygen species level, reduced glutathione content and the activities of superoxide dismutase and catalase, and upregulated the mRNA expression of selected oxidative stress-related genes. The obtained data demonstrated that TBECH exhibits immunotoxicity in macrophages, and will help to evaluate its health risks.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citocinas
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Espécies Reativas de Oxigênio
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Estresse Oxidativo
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Cicloexanos
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Retardadores de Chama
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Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Environ Toxicol
Ano de publicação:
2020
Tipo de documento:
Article