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Urinary N7-(1-hydroxy-3-buten-2-yl) guanine adducts in humans: temporal stability and association with smoking.
Jokipii Krueger, Caitlin C; Madugundu, Guru; Degner, Amanda; Patel, Yesha; Stram, Daniel O; Church, Timothy R; Tretyakova, Natalia.
Afiliação
  • Jokipii Krueger CC; Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
  • Madugundu G; Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
  • Degner A; Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
  • Patel Y; Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
  • Stram DO; Masonic Cancer Center, University of Minnesota, Minneapolis, MN.
  • Church TR; Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN.
  • Tretyakova N; Department of Preventative Medicine, School of Medicine, University of Southern California, Los Angeles, CA.
Mutagenesis ; 35(1): 19-26, 2020 02 13.
Article em En | MEDLINE | ID: mdl-31702786
1,3-Butadiene (BD) is a known human carcinogen found in cigarette smoke, automobile exhaust, and urban air. Workers occupationally exposed to BD in the workplace have an increased incidence of leukemia and lymphoma. BD undergoes cytochrome P450-mediated metabolic activation to 3,4-epoxy-1-butene (EB), 1,2,3,4-diepoxybutane (DEB) and 1,2-dihydroxy-3,4-epoxybutane (EBD), which form covalent adducts with DNA. We have previously reported a quantitative nanoLC/ESI+-HRMS3 method for urinary N7-(1-hydroxy-3-buten-2-yl) guanine (EB-GII) adducts as a mechanism-based biomarker of BD exposure. In the present study, the method was updated to include high throughput 96-well solid phase extraction (SPE) and employed to establish urinary EB-GII biomarker stability and association with smoking. Urinary EB-GII levels were measured bimonthly for 1 year in 19 smokers to determine whether single adduct measurement provides reliable levels of EB-GII in an individual smoker. In addition, association of EB-GII with smoking was studied in 17 individuals participating in a smoking cessation program. EB-GII levels decreased 34% upon smoking cessation, indicating that it is associated with smoking status, but may also originate from sources other than exposure to cigarette smoke.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 13_ODS3_tobacco_control / 2_ODS3 Base de dados: MEDLINE Assunto principal: Fumar / Adutos de DNA Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Mutagenesis Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 13_ODS3_tobacco_control / 2_ODS3 Base de dados: MEDLINE Assunto principal: Fumar / Adutos de DNA Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Mutagenesis Ano de publicação: 2020 Tipo de documento: Article