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Six-week inhalation of CdO nanoparticles in mice: The effects on immune response, oxidative stress, antioxidative defense, fibrotic response, and bones.
Tulinska, Jana; Masanova, Vlasta; Liskova, Aurelia; Mikusova, Miroslava Lehotska; Rollerova, Eva; Krivosikova, Zora; Stefikova, Kornelia; Uhnakova, Iveta; Ursinyova, Monika; Babickova, Janka; Babelova, Andrea; Busova, Milena; Tothova, Lubomira; Wsolova, Ladislava; Dusinska, Maria; Sojka, Martin; Horvathova, Mira; Alacova, Radka; Vecera, Zbynek; Mikuska, Pavel; Coufalik, Pavel; Krumal, Kamil; Capka, Lukas; Docekal, Bohumil.
Afiliação
  • Tulinska J; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Masanova V; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia. Electronic address: vlasta.masanova@szu.sk.
  • Liskova A; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Mikusova ML; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Rollerova E; Faculty of Public Health, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Krivosikova Z; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Stefikova K; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Uhnakova I; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Ursinyova M; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Babickova J; Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Spitalska 24, 813 72, Bratislava, Slovakia; Department of Clinical Medicine, University of Bergen, PO Box 7800, 5020, Bergen, Norway.
  • Babelova A; Cancer Research Institute, Biomedical Research Centre, Slovak Academy of Sciences, Dubravska cesta 9, 81439, Bratislava, Slovakia.
  • Busova M; Institute of Hygiene and Epidemiology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Katerinska 1660/32, 12108, Prague, Czech Republic.
  • Tothova L; Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Spitalska 24, 813 72, Bratislava, Slovakia.
  • Wsolova L; Faculty of Public Health, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Dusinska M; Norwegian Institute for Air Research, Health Effects Laboratory, PO Box 100, 2027, Kjeller, Norway.
  • Sojka M; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Horvathova M; Faculty of Medicine, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Alacova R; Faculty of Public Health, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
  • Vecera Z; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
  • Mikuska P; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
  • Coufalik P; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
  • Krumal K; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
  • Capka L; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
  • Docekal B; Institute of Analytical Chemistry of Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic.
Food Chem Toxicol ; 136: 110954, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31707033
Due to the growing number of applications of cadmium oxide nanoparticles (CdO NPs), there is a concern about their potential deleterious effects. The objective of our study was to investigate the effect of CdO NPs on the immune response, renal and intestine oxidative stress, blood antioxidant defence, renal fibrotic response, bone density and mineral content. Six-week-old female ICR mice were exposed to CdO NPs for 6 weeks by inhalation (particle size: 9.82 nm, mass concentration: 31.7 µg CdO/m3, total deposited dose: 0.195 µg CdO/g body weight). CdO NPs increased percentage of thymus CD3e+CD8a+ cells and moderately enhanced splenocyte proliferation and production of cytokines and chemokines. CdO NPs elevated pro-fibrotic factors (TGF-ß2, α-SMA and collagen I) in the kidney, and concentrations of AGEs in the intestine. The ratio of GSH and GSSG in blood was slightly reduced. Exposure to CdO NPs resulted in 10-fold higher Cd concentration in tibia bones. No differences were found in bone mass density, mineral content, bone area values, bone concentrations of Ca, P, Mg and Ca/P ratio. Our findings indicate stimulation of immune/inflammatory response, oxidative stress in the intestine, starting fibrotic response in kidneys and accumulation of CdO NPs in bones of mice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Tíbia / Fibrose / Estresse Oxidativo / Compostos de Cádmio / Nanopartículas Metálicas / Imunidade Celular Limite: Animals Idioma: En Revista: Food Chem Toxicol Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Tíbia / Fibrose / Estresse Oxidativo / Compostos de Cádmio / Nanopartículas Metálicas / Imunidade Celular Limite: Animals Idioma: En Revista: Food Chem Toxicol Ano de publicação: 2020 Tipo de documento: Article