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Real-Time NMR Spectroscopy for Studying Metabolism.
Alshamleh, Islam; Krause, Nina; Richter, Christian; Kurrle, Nina; Serve, Hubert; Günther, Ulrich L; Schwalbe, Harald.
Afiliação
  • Alshamleh I; Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue Str. 7, 60438, Frankfurt, Germany.
  • Krause N; Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue Str. 7, 60438, Frankfurt, Germany.
  • Richter C; Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue Str. 7, 60438, Frankfurt, Germany.
  • Kurrle N; German Cancer Consortium (DKTK) and DKFZ, 69120, Heidelberg, Germany.
  • Serve H; Department of Medicine 2, Hematology/Oncology, Goethe University, 60590, Frankfurt am Main, Germany.
  • Günther UL; Frankfurt Cancer Institute (FCI), 60590, Frankfurt am Main, Germany.
  • Schwalbe H; German Cancer Consortium (DKTK) and DKFZ, 69120, Heidelberg, Germany.
Angew Chem Int Ed Engl ; 59(6): 2304-2308, 2020 02 03.
Article em En | MEDLINE | ID: mdl-31730253
Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48 hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in-cell NMR spectroscopy experiments. We are able to monitor real-time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7 minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer-based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância Magnética / Metabolômica Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância Magnética / Metabolômica Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2020 Tipo de documento: Article