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Intravenous immunoglobulin therapy enhances suppressive regulatory T cells and decreases innate lymphoid cells in children with immune thrombocytopenia.
Wang, Shih-Chung; Yang, Kuender D; Lin, Ching-Yuang; Huang, Alex Y; Hsiao, Chien-Chou; Lin, Ming-Tsan; Tsai, Yi-Giien.
Afiliação
  • Wang SC; Division of Pediatric Hematology-Oncology, Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan.
  • Yang KD; Mackay Children's Hospital, and Institute of Biomedical Sciences, Mackay Medical College, Taipei, Taiwan.
  • Lin CY; Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan.
  • Huang AY; Clinical Immunological Center, China Medical University Hospital, Taichung, Taiwan.
  • Hsiao CC; Division of Pediatric Nephrology, Childrens' Hospital, China Medical University, Taichung, Taiwan.
  • Lin MT; Division of Pediatric Hematology-Oncology, Department of Pediatrics, UH Rainbow Babies and Children's Hospital; Case Western Reserve University School of Medicine, Cleveland, Ohio.
  • Tsai YG; Division of Pediatric Hematology-Oncology, Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan.
Pediatr Blood Cancer ; 67(2): e28075, 2020 02.
Article em En | MEDLINE | ID: mdl-31736241
ABSTRACT

BACKGROUND:

This study aimed to investigate the relationship between CD4+ regulatory T cells (Tregs) and innate lymphoid cells (ILCs) in children with primary immune thrombocytopenia (ITP) undergoing high-dose intravenous immunoglobulin (IVIG) therapy.

METHODS:

We enrolled a cohort of 30 children with newly diagnosed ITP and 30 healthy controls and collected blood samples for levels of Tregs, ILCs, relevant cytokines, and Treg suppression assay at the diagnosis, two days, four weeks, and one year (only platelet count) after high-dose IVIG treatment. IVIG partial responders was defined by a platelet count less than 100 × 109 /L at 12 months after IVIG treatment.

RESULTS:

Children with newly diagnosed ITP exhibited elevated levels of ILC1, ILC2, ILC3, Th17, myeloid dendritic cells (DCs), plasmacytoid DCs, and serum IFN-γ and IL-17A levels, accompanied by a decrease in IL-10-producing Tregs. High-dose IVIG therapy reversed these aberrations. Platelet counts positively correlated with Tregs (rho = 0.72) and negatively correlated with both ILC1 (rho = -0.49) and ILC3 (rho = -0.60) (P < 0.05). Significantly lower Tregs and higher ILC1, ILC3, DCs, and serum IL-17A levels were noted in the partial responders (n = 8) versus responders (n = 22; P < 0.05). We found that Tregs suppressed proliferation of ILCs and CD4+ T cells in CD25-depleted peripheral PBMCs and enhanced the apoptosis of CD4+ CD45RO+ T cells in vitro following IVIG therapy.

CONCLUSIONS:

Effective high-dose IVIG therapy for children with newly diagnosed ITP appears to result in the induction of Tregs, which suppresses ILC proliferation in vitro and is associated with platelet response.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos / Púrpura Trombocitopênica Idiopática / Imunoglobulinas Intravenosas / Linfócitos T Reguladores / Imunidade Inata Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Pediatr Blood Cancer Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos / Púrpura Trombocitopênica Idiopática / Imunoglobulinas Intravenosas / Linfócitos T Reguladores / Imunidade Inata Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Pediatr Blood Cancer Ano de publicação: 2020 Tipo de documento: Article