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miR­204 inhibits the osteogenic differentiation of mesenchymal stem cells by targeting bone morphogenetic protein 2.
Jiang, Xiaofeng; Zhang, Zuofu; Peng, Tao; Wang, Guangda; Xu, Qiang; Li, Guangrun.
Afiliação
  • Jiang X; Department of Joint Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
  • Zhang Z; Department of Joint Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
  • Peng T; Department of Orthopedics, Pingdu People's Hospital, Pingdu, Shandong 266700, P.R. China.
  • Wang G; Department of Joint Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
  • Xu Q; Department of Joint Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
  • Li G; Department of Spinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
Mol Med Rep ; 21(1): 43-50, 2020 01.
Article em En | MEDLINE | ID: mdl-31746352
Mesenchymal stem cells (MSCs) are used to investigate regeneration and differentiation. MicroRNA­204 (miR­204) in involved in the Runt­related transcription factor 2/alkaline phosphatase/bone morphogenic protein 2 (Runx2/ALP/BMP2) signaling pathway that regulates bone marrow mesenchymal stem cell (BMSC) differentiation; however, the mechanisms underlying the effects of miR­204 are yet to be determined. The aim of the present study was to investigate the effects of miR­204 on BMSC differentiation. BMSCs were derived from rat bone marrow. The expression levels of Runx2, ALP and BMP2 were measured via reverse transcription­quantitative polymerase chain reaction and western blot analyses following transfection of BMSCs with miR­204 agomir or BMP2 expression vector. The ability of the miR­204 gene to directly bind BMP2 mRNA was assessed using dual­luciferase assays. Ossification was measured via alizarin red stain assays. It was observed that the expression levels of Runx2 and ALP increased over time, whereas those of miR­204 decreased; additionally, miR­204 agomir upregulation inhibited the expression of Runx2, ALP and BMP2 in BMSCs. It was revealed that miR­204 directly interacted with BMP2 mRNA, and that transfection with miR­204 agomir suppressed ossification in BMSCs by targeting the BMP2/Runx2/ALP signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Transdução de Sinais / Diferenciação Celular / MicroRNAs / Proteína Morfogenética Óssea 2 / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Mol Med Rep Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Transdução de Sinais / Diferenciação Celular / MicroRNAs / Proteína Morfogenética Óssea 2 / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Mol Med Rep Ano de publicação: 2020 Tipo de documento: Article