NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.
J Cell Biol
; 219(1)2020 01 06.
Article
em En
| MEDLINE
| ID: mdl-31816056
ABSTRACT
The development of cerebral cortex requires spatially and temporally orchestrated proliferation, migration, and differentiation of neural progenitor cells (NPCs). The molecular mechanisms underlying cortical development are, however, not fully understood. The neural cell adhesion molecule (NCAM) has been suggested to play a role in corticogenesis. Here we show that NCAM is dynamically expressed in the developing cortex. NCAM expression in NPCs is highest in the neurogenic period and declines during the gliogenic period. In mice bearing an NPC-specific NCAM deletion, proliferation of NPCs is reduced, and production of cortical neurons is delayed, while formation of cortical glia is advanced. Mechanistically, NCAM enhances actin polymerization in NPCs by interacting with actin-associated protein profilin2. NCAM-dependent regulation of NPCs is blocked by mutations in the profilin2 binding site. Thus, NCAM plays an essential role in NPC proliferation and fate decision during cortical development by regulating profilin2-dependent actin polymerization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Córtex Cerebral
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Antígeno CD56
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Profilinas
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Neurogênese
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Células-Tronco Neurais
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2020
Tipo de documento:
Article