Simultaneous Enhancement of Thermostability and Catalytic Activity of a Metagenome-Derived ß-Glucosidase Using Directed Evolution for the Biosynthesis of Butyl Glucoside.

Yin, Bangqiao; Hui, Qinyan; Kashif, Muhammad; Yu, Ran; Chen, Si; Ou, Qian; Wu, Bo; Jiang, Chengjian

Yin, Bangqiao; Hui, Qinyan; Kashif, Muhammad; Yu, Ran; Chen, Si; Ou, Qian; Wu, Bo; Jiang, Chengjian.

Afiliação

Yin B; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Hui Q; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Kashif M; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Yu R; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Chen S; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Ou Q; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Wu B; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Jiang C; Department of chemical and biological engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China.

Int J Mol Sci ; 20(24)2019 Dec 10.

Article em En | MEDLINE | ID: mdl-31835569

Assuntos

Evolução Molecular Direcionada/métodos; Glucosídeos/metabolismo; beta-Glucosidase/química; beta-Glucosidase/metabolismo; Substituição de Aminoácidos; Proteínas de Bactérias/química; Proteínas de Bactérias/genética; Proteínas de Bactérias/metabolismo; Estabilidade Enzimática; Meia-Vida; Temperatura Alta; Metagenoma; Mutagênese Sítio-Dirigida; Termodinâmica; beta-Glucosidase/genética

Palavras-chave

butyl glucoside; directed evolution; metagenome-derived ß-glucosidase; site-directed mutagenesis; thermostability

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Glucosidase / Evolução Molecular Direcionada / Glucosídeos Idioma: En Revista: Int J Mol Sci Ano de publicação: 2019 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google