Toxic mechanism of eucalyptol and ß-cyclocitral on Chlamydomonas reinhardtii by inducing programmed cell death.
J Hazard Mater
; 389: 121910, 2020 05 05.
Article
em En
| MEDLINE
| ID: mdl-31879110
ABSTRACT
Eucalyptol and ß-cyclocitral are 2 main compounds in cyanobacterial volatile organic compounds and can poison other algae. To uncover the toxic mechanism of the 2 compounds, the cell growth, photosynthetic abilities, H2O2 production, caspase-like activities, nuclear variation and DNA laddering were investigated in Chlamydomonas reinhardtii treated with eucalyptol and ß-cyclocitral. Eucalyptol at ≥ 0.1 mM and ß-cyclocitral at ≥ 0.05 mM showed toxic effects on C. reinhardtii cells, and 1.2 mM eucalyptol and 0.4 mM ß-cyclocitral killed the whole of the cells during 24 h. During the death process, the photosynthetic pigment gradually degraded, and Fv/Fm gradually declined, indicating that the death is not a necrosis due to the gradual disappearance of the physiological process. In the treatments with 1.2 mM eucalyptol and 0.4 mM ß-cyclocitral, H2O2 content burst at 10 min and 30 min, respectively. Caspase-9-like and caspase-3-like were activated, and cell nucleuses concentrated firstly and then broke with prolonging the treatment time. Meanwhile, DNA showed laddering after 1 h, and was gradually cleaved by Ca2+-dependent endonucleases to mainly about 100-250 bp fragments. These hallmarks indicated that eucalyptol and ß-cyclocitral may poison other algal cells by inducing programmed cell death triggered by the increased H2O2.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Chlamydomonas reinhardtii
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Apoptose
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Diterpenos
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Aldeídos
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Eucaliptol
Idioma:
En
Revista:
J Hazard Mater
Ano de publicação:
2020
Tipo de documento:
Article