Structural characterization and α-glycosidase inhibitory activity of a novel polysaccharide fraction from Aconitum coreanum.
Carbohydr Polym
; 230: 115586, 2020 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-31887942
ABSTRACT
α-Glycosidase is an essential target for the management of postprandial serum glucose in diabetic patients. Therefore, the interest has been growing in the screening of α-glycosidase inhibitor from natural resource. In the present study, the structure and α-glycosidase inhibitory activity of a polysaccharide (named as ACPP-1) from Aconitum coreanum were investigated. Based on the results from high performance gel permeation chromatography, GC-MS and 1D/2D nuclear magnetic resonance spectroscopy, ACPP-1 was a highly-linear polysaccharide with a molecular weight of 34.0â¯kD and containing over 90 % of glucose. It was composed of (1â4)-α-d-Glcp and α-Araf. ACPP-1 exhibited a dose-dependent inhibitory eï¬ ;ect against α-glycosidase activity in vitro and the IC50 value was â¼0.8â¯mg/mL. In oral starch tolerance test, treatment with ACPP-1 (800â¯mg/kg) significantly improved the starch tolerance in mice. Taken together, this study provided a potential intervention and management for postprandial hyperglycemia by the polysaccharide fraction from A. coreanum.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Aconitum
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Alfa-Glucosidases
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Inibidores de Glicosídeo Hidrolases
Limite:
Animals
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Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2020
Tipo de documento:
Article