H,K-ATPase type 2 regulates gestational extracellular compartment expansion and blood pressure in mice.
Am J Physiol Regul Integr Comp Physiol
; 318(2): R320-R328, 2020 02 01.
Article
em En
| MEDLINE
| ID: mdl-31913688
The modifications of the hemodynamic system and hydromineral metabolism are physiological features characterizing a normal gestation. Thus, the ability to expand plasma volume without increasing the level of blood pressure is necessary for the correct perfusion of the placenta. The kidney is essential in this adaptation by reabsorbing avidly sodium and fluid. In this study, we observed that the H,K-ATPase type 2 (HKA2), an ion pump expressed in kidney and colon and already involved in the control of the K+ balance during gestation, is also required for the correct plasma volume expansion and to maintain normal blood pressure. Indeed, compared with WT pregnant mice that exhibit a 1.6-fold increase of their plasma volume, pregnant HKA2-null mice (HKA2KO) only modestly expand their extracellular volume (×1.2). The renal expression of the epithelial Na channel (ENaC) α- and γ-subunits and that of the pendrin are stimulated in gravid WT mice, whereas the Na/Cl- cotransporter (NCC) expression is downregulated. These modifications are all blunted in HKA2KO mice. This impeded renal adaptation to gestation is accompanied by the development of hypotension in the pregnant HKA2KO mice. Altogether, our results showed that the absence of the HKA2 during gestation leads to an "underfilled" situation and has established this transporter as a key player of the renal control of salt and potassium metabolism during gestation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Volume Plasmático
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Potássio
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Sódio
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Pressão Sanguínea
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ATPase Trocadora de Hidrogênio-Potássio
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Rim
Limite:
Animals
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Pregnancy
Idioma:
En
Revista:
Am J Physiol Regul Integr Comp Physiol
Ano de publicação:
2020
Tipo de documento:
Article