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A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus.
Reid, Peter; Heng, Nicholas C K; Hale, John D; Krishnan, Deepti; Crane, Julian; Tagg, John R; Milne, Trudy J.
Afiliação
  • Reid P; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
  • Heng NCK; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
  • Hale JD; Blis Technologies Limited, 81 Glasgow Street, South Dunedin 9012, New Zealand.
  • Krishnan D; Blis Technologies Limited, 81 Glasgow Street, South Dunedin 9012, New Zealand.
  • Crane J; School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand.
  • Tagg JR; Blis Technologies Limited, 81 Glasgow Street, South Dunedin 9012, New Zealand.
  • Milne TJ; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand. Electronic address: trudy.milne@otago.ac.nz.
J Microbiol Methods ; 170: 105837, 2020 03.
Article em En | MEDLINE | ID: mdl-31923428
ABSTRACT
In order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations targeting genes of the locus encoding the lantibiotic bacteriocin salivaricin B.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carga Bacteriana / Streptococcus salivarius Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carga Bacteriana / Streptococcus salivarius Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2020 Tipo de documento: Article