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Evaluation of a novel inactivated vaccine against duck circovirus in muscovy ducks.
Zhaolong, Li; Guanghua, Fu; Zhihua, Feng; Jianhua, Chen; Shaohua, Shi; Rongchang, Liu; Longfei, Cheng; Hongmei, Chen; Chunhe, Wan; Yu, Huang.
Afiliação
  • Zhaolong L; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China. Electronic address: 497377512@qq.com.
  • Guanghua F; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Zhihua F; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China. Electronic address: 5742411833@qq.com.
  • Jianhua C; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China. Electronic address: 6958904593@qq.com.
  • Shaohua S; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Rongchang L; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Longfei C; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Hongmei C; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Chunhe W; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
  • Yu H; Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350013, China; Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian Province, 350013, China; Fujian Animal Disease Control Technology Center,
Vet Microbiol ; 241: 108574, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31928707
Duck circovirus (DuCV), an immunosuppressive pathogen, causes serious damage to waterfowls worldwide. A highly efficient vaccine would play a crucial role in preventing DuCV infections in the waterfowl breeding industry. However, to date, there is a dearth of commercial vaccines owing to the lack of a cell culture system for propagating the requisite virus amounts in vitro. In this study, we isolated DuCVs from Muscovy ducks, helped them proliferate using peripheral blood mononuclear cells (PBMCs), and developed an inactivated vaccine. Muscovy ducks vaccinated with the inactivated vaccine had higher neutralizing antibody titers than the control ducks and higher protection in the challenge experiment (as assessed by weight measurement). Moreover, the inactivated vaccine did not cause feather abnormalities, growth repression, and dwarf syndrome; likewise, lesions and lymphocyte apoptosis in the bursa of Fabricius, spleen, and thymus were not observed. Significantly lower virus shedding from the inactivated vaccine was detected up to 42 days post-inoculation. Together, these results suggest that the inactivated DuCV vaccine can induce a high immune response, is relatively safer for Muscovy ducks, and thus it is a protective vaccine candidates against DuCV infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Vacinas Virais / Circovirus / Infecções por Circoviridae / Patos Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Vacinas Virais / Circovirus / Infecções por Circoviridae / Patos Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 2020 Tipo de documento: Article