The activation of NF-κB and MAPKs signaling pathways of RAW264.7 murine macrophages and natural killer cells by fucoidan from Nizamuddinia zanardinii.
Int J Biol Macromol
; 148: 56-67, 2020 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-31953171
ABSTRACT
Polysaccharides from Nizamuddinia zanardinii were extracted using water at elevated temperature and fractionated by a DEAE Sepharose FF column yielding four fractions (F1-F4). Crude and fractions were composed of neutral sugars (50.8-57.4%), proteins (10.8-18.1%), sulfates (7.5-17.3%) and uronic acids (3.5-7.7%). Various levels of galactose (13.4-44.4%), fucose (34.1-40.1%), mannose (14.1-33.2%) and xylose (7.4-15.2%) formed the building blocks of the polysaccharide structures. The weight average molecular weights (Mw) of polysaccharides varied between 40.3 and 1254.4 × 103 g/mol. F3 polysaccharide was the most active fraction stimulating RAW264.7 murine macrophage cells to secrete NO, TNF-α, IL-1ß and IL-6, and activating NK cells to release TNF-α, INF-γ, granzyme-B, perforin, NKG2D and FasL through NF-κB and MAPKs signaling pathways. Highly-branched F3 polysaccharide mainly consisted of (1 â 2)-Fucp, (1 â 2,3)-Manp, (1 â 3)-Galp, (1 â 2)-Manp, (1 â 3)-Manp, (1 â 2,3,4)-Manp and (1 â 2,3,6)-Manp residues with great amount of (â1)-Fucp and (â1)-Xylp. Sulfates substituted at C-2 of fucose and galactose residues. Overall, fucoidan from N. zanardinii showed immense potency in boosting immune system through macrophages and NK cells activations and therefore suitable for further exploration in immune-mediated biomedical applications.
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Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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NF-kappa B
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Sistema de Sinalização das MAP Quinases
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Phaeophyceae
Limite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2020
Tipo de documento:
Article