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Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States are Maintained in 3D Culture.
D'Souza, Rochelle C J; Offenhäuser, Carolin; Straube, Jasmin; Baumgartner, Ulrich; Kordowski, Anja; Li, Yuchen; Stringer, Brett W; Alexander, Hamish; Lwin, Zarnie; Inglis, Po-Ling; Jeffree, Rosalind L; Johns, Terrance G; Boyd, Andrew W; Day, Bryan W.
Afiliação
  • D'Souza RCJ; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Offenhäuser C; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Straube J; Immunology Department, QIMR Berghofer Medical Research Institute, Gordon and Jessie Gilmour Leukaemia Research Laboratory, Brisbane, QLD 4006, Australia.
  • Baumgartner U; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Kordowski A; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Li Y; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Stringer BW; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Alexander H; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Lwin Z; Royal Brisbane and Women's Hospital, Brisbane, QLD 4006, Australia.
  • Inglis PL; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Jeffree RL; Royal Brisbane and Women's Hospital, Brisbane, QLD 4006, Australia.
  • Johns TG; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
  • Boyd AW; Royal Brisbane and Women's Hospital, Brisbane, QLD 4006, Australia.
  • Day BW; Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Sid Faithfull Brain Cancer Laboratory, Brisbane, QLD 4006, Australia.
Cells ; 9(2)2020 01 21.
Article em En | MEDLINE | ID: mdl-31973233
Glioblastoma (GBM) is a treatment-refractory central nervous system (CNS) tumour, and better therapies to treat this aggressive disease are urgently needed. Primary GBM models that represent the true disease state are essential to better understand disease biology and for accurate preclinical therapy assessment. We have previously presented a comprehensive transcriptome characterisation of a panel (n = 12) of primary GBM models (Q-Cell). We have now generated a systematic, quantitative, and deep proteome abundance atlas of the Q-Cell models grown in 3D culture, representing 6167 human proteins. A recent study has highlighted the degree of functional heterogeneity that coexists within individual GBM tumours, describing four cellular states (MES-like, NPC-like, OPC-like and AC-like). We performed comparative proteomic analysis, confirming a good representation of each of the four cell-states across the 13 models examined. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified upregulation of a number of GBM-associated cancer pathway proteins. Bioinformatics analysis, using the OncoKB database, identified a number of functional actionable targets that were either uniquely or ubiquitously expressed across the panel. This study provides an in-depth proteomic analysis of the GBM Q-Cell resource, which should prove a valuable functional dataset for future biological and preclinical investigations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glioblastoma / Técnicas de Cultura de Células / Proteômica Limite: Humans Idioma: En Revista: Cells Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glioblastoma / Técnicas de Cultura de Células / Proteômica Limite: Humans Idioma: En Revista: Cells Ano de publicação: 2020 Tipo de documento: Article