Shewanella polaris sp. nov., a psychrotolerant bacterium isolated from Arctic brown algae.
Int J Syst Evol Microbiol
; 70(3): 2096-2102, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-31999242
ABSTRACT
A Gram-stain-negative, facultatively anaerobic, flagellated and rod-shaped bacterium, designated strain SM1901T, was isolated from a brown algal sample collected from Kings Bay, Svalbard, Arctic. Strain SM1901T grew at -4â30 °C and with 0-7.0â% (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed DNA and Tween 80. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SM1901T was affiliated with the genus Shewanella, showing the highest sequence similarity to the type strain of Shewanella litoralis (97.5%), followed by those of Shewanella vesiculosa, Shewanella livingstonensis and Shewanella saliphila (97.3â% for all three). The major cellular fatty acids were summed feature 3 (C16ââ1 ω7Ñ and/or C16ââ1 ω6Ñ), C16ââ0, C18ââ0, iso-C15ââ0 and C17ââ1 ω8Ñ and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The respiratory quinones were ubiquinones Q-7, Q-8, menaquinones MK-7(H) and MK-8. The genome of strain SM1901T was 4648537 nucleotides long and encoded a variety of cold adaptation related genes, providing clues for better understanding the ecological adaptation mechanisms of polar bacteria. The genomic DNA G+C content of strain SM1901T was 40.5 mol%. Based on the polyphasic evidence presented in this paper, strain SM1901T was considered to represent a novel species, constituting a novel psychrotolerant lineage out of the known SF clade encompassed by polar Shewanella species, within the genus Shewanella, for which the name Shewanella polaris sp. nov. is proposed. The type strain is SM1901T (=KCTC 72047T=MCCC 1K03585T).
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Shewanella
/
Phaeophyceae
País/Região como assunto:
Europa
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Ano de publicação:
2020
Tipo de documento:
Article