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Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells.
Wang, Xue; Wang, Ziming; Adeniran, S O; Huang, Fushuo; Ma, Mingjun; Zhang, Han; Li, Xiaoyu; Zheng, Peng; Zhang, Guixue.
Afiliação
  • Wang X; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Wang Z; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Adeniran SO; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Huang F; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Ma M; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Zhang H; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Li X; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Zheng P; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • Zhang G; Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China; and Corresponding author. Email: Gxzhang@neau.edu.cn.
Reprod Fertil Dev ; 32(5): 522-530, 2020 Mar.
Article em En | MEDLINE | ID: mdl-32023428
ABSTRACT
The gap junction protein connexin (Cx) 43 between adjacent Sertoli cells (SCs) is the main testicular factor regulating the growth and development of SCs, and plays a vital role in controlling cell differentiation and maturation. However, the endogenous testicular factors that regulate Cx43 and the downstream signalling pathways that mediate Cx43-dependent SC differentiation are unclear. In this study, high-purity SCs were isolated from newborn calves' testes by differential adherence. The SCs were then cultured invitro and treated with short interference RNA to knockdown endogenous Wilms' tumour 1 (WT1). In WT1-knockdown SCs, Cx43 expression was upregulated. To elucidate the intracellular signalling mechanism of Cx43 in the differentiation and maturation of immature SCs, SCs were treated simultaneously with non-canonical Wnt signalling inhibitors CCG-1423 and GO-6983; in these SCs, Cx43 expression was upregulated. Together, these data indicate that WT1 negatively regulates the expression of Cx43 produced from SCs via a non-canonical Wnt signalling pathway in cultured bovine SCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Sertoli / Conexina 43 / Proteínas WT1 / Via de Sinalização Wnt Limite: Animals Idioma: En Revista: Reprod Fertil Dev Ano de publicação: 2020 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Sertoli / Conexina 43 / Proteínas WT1 / Via de Sinalização Wnt Limite: Animals Idioma: En Revista: Reprod Fertil Dev Ano de publicação: 2020 Tipo de documento: Article