Optimizing red blood cell protein extraction for biomarker quantitation with mass spectrometry.

Klatt, Stephan; Roberts, Anne; Lothian, Amber; Masters, Colin L; Cappai, Roberto; Fowler, Christopher; Roberts, Blaine R

Klatt, Stephan; Roberts, Anne; Lothian, Amber; Masters, Colin L; Cappai, Roberto; Fowler, Christopher; Roberts, Blaine R.

Afiliação

Klatt S; The Florey Institute of Neuroscience and Mental Health, Melbourne Dementia Research Centre, The University of Melbourne, 30 Royal Parade, Parkville, Victoria, 3052, Australia.
Roberts A; Cooperative Research Centre for Mental Health, Parkville, Victoria, 3052, Australia.
Lothian A; The Florey Institute of Neuroscience and Mental Health, Melbourne Dementia Research Centre, The University of Melbourne, 30 Royal Parade, Parkville, Victoria, 3052, Australia.
Masters CL; Cooperative Research Centre for Mental Health, Parkville, Victoria, 3052, Australia.
Cappai R; The Florey Institute of Neuroscience and Mental Health, Melbourne Dementia Research Centre, The University of Melbourne, 30 Royal Parade, Parkville, Victoria, 3052, Australia.
Fowler C; Cooperative Research Centre for Mental Health, Parkville, Victoria, 3052, Australia.
Roberts BR; The Florey Institute of Neuroscience and Mental Health, Melbourne Dementia Research Centre, The University of Melbourne, 30 Royal Parade, Parkville, Victoria, 3052, Australia.

Anal Bioanal Chem ; 412(8): 1879-1892, 2020 Mar.

Article em En | MEDLINE | ID: mdl-32030493

Assuntos

Proteínas Sanguíneas/isolamento & purificação; Eritrócitos/metabolismo; Espectrometria de Massas/métodos; Biomarcadores/sangue; Cromatografia Líquida/métodos; Congelamento; Ensaios de Triagem em Larga Escala; Humanos; alfa-Sinucleína/sangue

Palavras-chave

Erythrocytes; HemoVoid™; Proteomics; Red blood cells; Sodium deoxycholate; Urea

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteínas Sanguíneas / Eritrócitos Limite: Humans Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2020 Tipo de documento: Article

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