Simultaneous determination of six alkaloids in tobacco and tobacco products by direct analysis of real-time triple quadrupole mass spectrometry with a modified pretreatment method.
J Sep Sci
; 43(8): 1603-1613, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-32034866
In order to determine six alkaloids (mass fraction) of nicotine, nornicotine, myosmine, anatabine, anabasine, and nicotyrine in tobacco and tobacco products quickly, accurately, and simultaneously, a novel method based on direct analysis of real-time model in situ ionization technique combined tandem mass spectrometry with a modified sample pretreatment was established, in which experimental parameters such as the type and amount of extraction solvent and injection rate were optimized, respectively. The samples of five commercial cigarettes and five kinds of tobacco leaves were analyzed by the established method, and the determined values were compared with those obtained using a gas chromatography with mass spectrometry method: (1) Under optimized conditions (30 mL ultrapure water as extraction solvent and with extraction rate of 0.6 mm/s), analysis could be completed within 10 min. (2) The linear range of the method was 0.002-2000 µg/g with R 2 = 0.9957 , the recovery ranged from 86.8 to 105.6%, and the limit of detection and the limit of quantification were 0.004-0.835 µg/g and 0.013-2.787 µg/g, respectively. (3) The relative standard deviation between direct analysis of real-time method and the gas chromatography with mass spectrometry method was 0.34-8.83%. The established method is rapid, reliable, and suitable for the ultrafast determination of six alkaloids in tobacco and tobacco products.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
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Alcaloides
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Produtos do Tabaco
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2020
Tipo de documento:
Article