Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics.
Sensors (Basel)
; 20(5)2020 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-32151107
ABSTRACT
A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer-poly-(2,6-di([2,2'-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on low temperature cofired ceramics technology (LTCC). The detection procedure was based on the oxidation of the substrate, i.e., in the presence of the enzyme. An alternative enzyme-free system utilized the formation of a colorful complex between Fe2+ ions and epinephrine molecules. With the optimized conditions, the analytical performance illustrated high sensitivity and selectivity in a broad linear range with a detection limit of 0.14-2.10 nM. Moreover, the strategy was successfully used for an EP injection test with labeled pharmacological samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2020
Tipo de documento:
Article